Animal Biotechnology: Syllabus

 

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UNIT-I

1) History and development of animal biotechnology:

1. Introduction
2. Definition
3. Contribution of various Scientist
4. Steps/Mile stones in development of Animal Biotechnology.

2) Basic techniques in animal cell culture:

1. Dissociation/ Disaggregation Techniques.
2. Primary and secondary cell culture.
3. Cell line and their types.

3) Oocyte collection and maturation:

1. Collection methods and in vitro culture
2. Maintenance of Oocyte.

4) Sperm Preparation:

1. Collection of sperms
2. Evaluation
3. Scaling up and preservation.

5) In vitro Fertilization:

1. Causes of infertility
2. Fusion of sperms and In vitro culture Oocyte
3. Zygote and Embryo growth and development

6) Cryopreservation of Oocyte, sperm and embryos:

1. Preparation
2. Methods of freezing
3. Storage
4. Thawing and reculturing.

7) Embryo transfer technology:

1. Artificial insemination.
2. Freezing techniques.
3. Selection of Donor and recipients.
4. Super ovulation.
5. Estrous synchronization.
6. Applications of ETT.

UNIT-II

1) Breeds of livestock and their characteristics:

1. Classification.
2. Indian and exotic breeds of Cattle, Buffalo, Sheep, Goat and Poultry Bird. 
3. Their origin and distribution. 
4. Distinguishing characteristics and their production.

2) Marker assisted breeding of livestock:

1. Marker assisted selection.
2. Advantages-limitations.
3. Applications in Animal Breeding.

3) Introduction to Animal Genomics:

1. Definition.
2. Gene isolation, Sequencing. 
3. Applications of molecular markers such as RFLP, RAPD, SSRs. 
4. QTL, SNP, STR. 
5. Mitochondrial DNA polymorphism

4) Rumen and its environment:

1. Rumen micro flora.
2. Rumen microbes- role of rumen microbes in digestion. 
3. Genetic manipulation of rumen microbes for better utilization of feed.

5) Introduction to Nutrigenomics:

1. Concept of Nutrigenomics.
2. Role of nutrition on gene expression.
3. Applications of nutrigenomics. 

6) Milk Biome:

1. Terminology.
2. Types of microbes present in milk.
3. Bacterial load during different lactation stages. 
4. Manipulation of lactation by biotechnological tools.

7) Application of biotechnology in meat and meat product:

1. Growth factors related genes.
2. Transgenic animals for the meat production.

UNIT-III

1) Genome and protein based diagnostics of important animal diseases:

1. FMD: Etiology. Symptoms, pathogenesis, Diagnosis and treatment.
2. Brucellosis: Etiology. Symptoms, pathogenesis, Diagnosis and treatment.
3. PPR: Etiology. Symptoms, pathogenesis, Diagnosis and treatment.
4. Mastitis: Etiology. Symptoms, pathogenesis, Diagnosis and treatment.
5. Blue tongue: Etiology. Symptoms, pathogenesis, Diagnosis and treatment.
6. Newcastle disease: Etiology. Symptoms, pathogenesis, Diagnosis and treatment.

2) Introduction to vaccinology:

1. History, Development of vaccines.
2. Types of vaccines:
1. Live attenuated vaccines.
2. Killed vaccines.
3. Cell culture based vaccines. 
4. Recombinant vaccines.